Interaction of Organophosphorus Pesticides with DNA Nucleotides on a Boron‐Doped Diamond Electrode
نویسندگان
چکیده
Eletrodo de diamante foi usado para avaliar a interação dos nucleotídeos guanosina monofosfato (GMP) e adenosina monofosfato (AMP) com os pesticidas clorpirifós, metamidofós e monocrotofós. Observaram-se alterações nas correntes e potenciais de picos dos voltamogramas dos nucleotídeos na presença dos pesticidas dependendo da concentração de pesticida (de 5,0 × 10 a 5,0 × 10 mol L) e do tempo de interação (de 1 min a 4 h). Isto é provavelmente devido à ligação dos pesticidas às bases nitrogenadas presentes nos nucleotídeos, o que poderia levar a problemas na replicação do DNA e nas funções biológicas de nucleotídeos. Os pesticidas apresentaram maior interação com AMP que com GMP. Estudos sobre a interação de DNA 50 μg mL com os pesticidas (de 30 min até 4 h, e de 1,0 × 10 a 6,0 × 10 mol L) não revelaram picos relativos à abertura da dupla hélice ou desenrolamento do DNA.
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